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TRANSACTIVATION RESPONSE OF VDRB1 IS PROMOTER- CELL- AND LIGAND-SPECIFIC C Fong, LM Esteban, SJ Allison, JL Flanagan, D Amr * , C Liddle * , JA Eisman & EM Gardiner Bone and Mineral Research Program, Garvan Institute of Medical Research, Sydney, NSW * Department of Clinical Pharmacology and Toxicology, Westmead Hospital, Westmead, NSW The vitamin D receptor VDR ; mediates the effects of 1, 25 OH ; 2D3, the active form of vitamin D. The human VDRB1 isoform differs from the originally described VDRA by an N-terminal extension of 50 amino acids. We investigated cell-, promoterand ligand-specific transactivation by VDRB1. Relative activities of the VDR isoforms on two cytochrome P450 gene promoters, CYP24 and CYP3A4, were studied in COS1 and HEK293 cell lines. Transactivation was induced by 1, 25 OH ; 2D3 or the secondary bile acid, lithocholic acid LCA ; , a recently reported VDR activator.